To start with, they removed the animal's skin. Then, instead of using an electrical field to clear the lipids, they pumped detergent around the entire mouse through its own circulatory systems. By doing without the electric field, the team avoided tissue damage. The minimal swelling that occurred when the gel was absorbed was constrained by animal's bones and musculature.
Using this technique, Gradinaru's team was able to render peripheral organs transparent within two days and a whole mouse transparent within two weeks.
New Scientist: Detergent treatment turns a mouse see-through
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